A Novel Strategy to Enhance Antioxidant Content in Saccharomyces Cerevisiae Based on Oxygen Pressure - CentraleSupélec Access content directly
Journal Articles Bioengineering Year : 2023

A Novel Strategy to Enhance Antioxidant Content in Saccharomyces Cerevisiae Based on Oxygen Pressure

Abstract

Antioxidant foods represent a potent lever to improve diets while creating value. Yet, their cultivation is often tied to a specific area and climate, limiting availability and increasing market cost. Therefore, microorganism-based antioxidant production emerges as a promising technology to solve these problems. In this view, a novel process was investigated for antioxidant accumulation in yeast culture. S. cerevisiae cells were exposed to various hyperbaric air conditions from 1 to 9 bar (A). Yeast cultures exhibited an increased reactive oxygen species content, which induced oxidative defense expression. After a few hours, reactive oxygen species levels decreased while antioxidant contents remained high, leading to a net increase in antioxidant power. At 6 bar (A), yeast achieved the highest net antioxidant power (phenolics content +48.3 ± 18.6 %, reducing power +120 ± 11.4 %) with an acceptable growth rate (0.27 h −1). Regarding time evolution, a 2 h exposure seems to be the optimum: cells have the lowest reactive oxygen species level while their antioxidant power is increased. From a biotechnological perspective, this finding highlights air pressure as an antioxidant-manipulating stress strategy. Moreover, the proposed process led to a patent that could potentially reduce energy and chemical consumption in such antioxidant accumulation processes.
Fichier principal
Vignette du fichier
bioengineering-10-00246.pdf (3.47 Mo) Télécharger le fichier
Origin Files produced by the author(s)

Dates and versions

hal-03986356 , version 1 (13-02-2023)

Identifiers

Cite

Na Cui, Patrick Perré, Emilie Michiels, Victor Pozzobon. A Novel Strategy to Enhance Antioxidant Content in Saccharomyces Cerevisiae Based on Oxygen Pressure. Bioengineering, 2023, 2 (10), pp.246. ⟨10.3390/bioengineering10020246⟩. ⟨hal-03986356⟩
15 View
21 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More